| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Richardson, Davia K (12)
|
MRYMID |
33.10 |
33.51 |
0.41 |
| 2 |
Sabol, Mackinzie I (12)
|
MRBGNW |
33.30 |
33.77 |
0.47 |
| 3 |
Lombardo, Kayla (12)
|
MRYMID |
33.97 |
35.12 |
1.15 |
| 4 |
Aikman, Saskia M (12)
|
MRYMID |
37.48 |
35.39 |
-2.09 |
| 5 |
Coulthard, Tamsin A (12)
|
MRYMID |
36.44 |
35.92 |
-0.52 |
| 6 |
Plawiak, Emilia A (11)
|
MRYMID |
35.07 |
36.10 |
1.03 |
| 7 |
McGourty, Megan R (11)
|
MRBGNW |
36.50 |
36.32 |
-0.18 |
| 8 |
Coppola, Sophia M (12)
|
MRBGNW |
37.02 |
36.34 |
-0.68 |
| 9 |
Valenzuela, Isabel A (12)
|
MRBGNW |
36.28 |
36.48 |
0.20 |
| 10 |
Gies, Caroline C (11)
|
CTZEUS |
36.68 |
36.49 |
-0.19 |
| 11 |
Redozubova, Sofiia (12)
|
CTZEUS |
40.52 |
40.81 |
0.29 |
| 12 |
Lingeza, Angelina (11)
|
MRYMID |
42.68 |
40.93 |
-1.75 |
| 13 |
Sorrells, Sienna R (11)
|
CTZEUS |
48.92 |
45.07 |
-3.85 |
| 14 |
Oh, Madeline J (11)
|
MRBGNW |
49.05 |
45.48 |
-3.57 |
| 15 |
Goldstein, Sabrina G (11)
|
CTZEUS |
46.44 |
45.54 |
-0.90 |
| 16 |
Hara, Alina (12)
|
MRYMID |
47.29 |
48.31 |
1.02 |
| 17 |
Higgins, Alice B (11)
|
MRBGNW |
50.09 |
51.14 |
1.05 |
| 18 |
Lane, Caroline G (11)
|
MRBGNW |
52.16 |
52.15 |
-0.01 |